Research Paper

Study Of Some Biochemical Variables Of Cardiovascular Patients And Effect Of Some Prodrugs On The Activity Of Some Enzymes In Vivo And In Vitro

Abstract :

Several studies have shown the risk factors associated cardiovascular disease (CVD). This research studies some biochemical variables of Cardiovascular patients and effect of some prodrugs on the activity of some enzymes in vivo and in vitro . in this study we estimate some biomarker for cardiovascular patients CK-MB ,LDH ,GPT ,GOT ,Cholesterol ,TG ,HDL ,LDL, VLDL, MDA ,Vitamin C and Vitamin E. Blood samples were collected from 50 cardiovascular patients (25 males and 25 females) and 50 healthy control (25 males and 25 females) from Tikrit hospital, Tikrit city Iraq. Ages of the involved subject range were from 33 to 65 years. Our result show a highly significant increase (p < 0.005) in the serum levels of CK-MB , LDH , cholesterol, triglyceride , HDL-cholesterol , GOT ,GPT, MDA in comparison with the control group and significant decrease in Alb ,HDL-Cholesterol ,Vitamin C and vitamin E in comparison with the control . No significant differences between gender (males and females) and no significant differences between ages. Inhibitory effect was study of some prodrugs on CK-MB and LDH activities in serum . The results revealed that the prodrugs have an inhibition effect on the CK-MB and LDH activities with higher inhibition range. The inhibition increased with the increasing concentration of prodrugs while the type of inhibitor showed noncompetitive inhibition. This study was conducted (in vivo) for Ascorbic acid prodrug )Ascorbic acid Indomethacine( (1) and Glucose prodrug (glucose Ibuprofe) (2). The concentration of Ibuprofen and Indomethacine was measured in serum after (2, 3, 4, 6, 8, 10) hours of giving a dose to the animal. The results showed that the concentration of Ibuprofen and Indomethacine were the highest after six hours of giving the animal dose. Biochemical study showed inhibition in enzyme activity for prodrugs1 and 2.

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