Comparison of Scleral Thickness and Fibroblast in Form Deprivation Animal Model (Experimental Study of Wistar Rat)
Rini Kusumawar Dhany
Background Form deprivation (FD) is an effective approach to induce animal models of myopia. In this investigation deprived of form vision compared to the fellow, non-deprived eye by histopathology of scleral thickness and fibroblast. Methods Ten of male three-weeks-old wistar rat weighing 200300 g were obtained from the Airlangga University Laboratory Animal Centre (Surabaya, Indonesia). All animals were examined clinically for confirming the corneal transparency of each eye and no injuries or infections of the eyes. Refractive errors were induced monocularly by covering right eye with adhesive tape in one group for 4 weeks which was named the FDM group. After enucleated under deep anaesthesia, histopathology of scleral thickness and fibroblast were observed. Results There were no differences in baseline axial length variance among the groups (F = 1.006, P = .413). Statistical analysis using t-test, scleral thickness in the treatment group was reduced compared with the control group but the difference was not statistically significant (p =0.443; p > 0.05). Scleral fibroblast in the treatment group was reduced compared with the control group but the difference was not statistically significant (p= 0.990; p > 0.05). Logistic regression analysis showed that association of form deprivation myopia, scleral thickness and scleral fibroblast was not significant (p=0.997) Conclusion The scleral thickness and scleral fibroblast of our study were reduced but not significant in FDM group compared with control group. These results not validate the FDM model, further study with more samples and longer FDM periods is necessary.
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