Original Research Paper

A Comparative Evaluation Of The Antibacterial Efficacy Of Various Root Canal Irrigants Against E. Fecalis : An In-vitro Study

Abstract :

The aim of endodontic treatment is to clean the root canal system including all vital and necrotic tissues, microorganisms, and its byproducts. The root canal system anatomy is extremely complex and diverse making it difficult to clean and shape it effectively. So, irrigation and disinfection of root canals plays a very important role in the success of endodontic treatment. The aim of this study was to evaluate & compare antimicrobial efficacy of 3.8% Silver Diamine Fluoride, 5.25% Sodium Hypochlorite, 100mg/ml Vitex Negundo Linn , 0.2 % Nano Chitosan , 0.9% Normal Saline and 2% Chlorhexidine against Enterococcus faecalis as root canal irrigant.Sixty single rooted human permanent teeth were taken and decoronated to standardize the canal length. After biomechanical preparation, teeth were inoculated with E.faecalis & randomly divided into 6 groups and the final irrigation was carried out with tested irrigants. Group A(n = 10): 0.9% Normal Saline(control); Group B(n = 10): 3.8% Silver Diamine Fluoride; Group C(n = 10): 5.25% Sodium hypochlorite; Group D(n = 10): 100mg/ml vitex negundo linn; Group E(n=10): 0.2% Nano Chitosan and Group F(n=10): 2% Chlorhexidine.The obtained constituent was cultured on agar plates & the number of CFUs (Colony forming units) per plate was determined using a digital colony counter, and statistically analysed using one-way ANOVA followed by post hoc Tukey test.5.25% Sodium Hypochlorite showed maximum antimicrobial activity against E. faecalis when compared to 3.8% Silver Diamine Fluoride, 100mg/ml Vitex Negundo Linn , 0.2 % Nano Chitosan , 0.9% Normal Saline and 2% Chlorhexidine.The present in-vitro study indicates that all the tested have exhibited effective antimicrobial efficacy against E. faecalis, with 5.25% Sodium Hypochlorite(Group C) found to be most effective against E. faecalis followed by the other irrigants.

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