Original Research Paper

Hemoglobin Estimation By Using Copper Sulphate Method

Abstract :

The use of the copper sulfate method is based on the fact that when a drop of whole blood is allowed to fall into the copper sulfate solution, there is formed an insoluble copper proteinate, and the behavior of this single drop of blood in the solution will determine the rejection or the acceptance of the blood donor. This procedure of Hemoglobin Estimation holds a great relevance in blood bank as it is cheap and gives fast result.PURPOSEHemoglobin estimation is done for every donor who presents himself / herself for blood donation to select healthy blood donor SCOPE Selection of healthy blood donors provides better post transfusion hemoglobin increment resulting in better oxygen carrying capacity as well as prevents donor to be anemic after donation. RESPONSIBILITY Responsibilitiesi.Check the quantity of copper sulfate stock solution every day at 5:00 pm, if stock is insufficient; prepare stock solution for next day. Preparation of fresh copper sulfate working solution every day in the morning.ii.Standardization of copper sulfate solution iii.Take 50 ml of solution and change after performing 20 25 tests.iv.Dispose used solution as biohazardous material because of blood in the container.v.In case of doubtful result confirm by automated sysmex-1000 prior to accepting or deferring the donor.Responsible person Designated technician and / or Nursing staff

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