Original Research Paper

Isolation, Identification And Antimicrobial Susceptibility Testing Of Escherichia Coli Isoleted From Raw Cow Milks Of Borena Breed Dairy Farms In Haramaya University, Eastern Ethiopia

Umer Seid, Chala Mohammed, Munera Ahmednur, Serkalem Haile, Abdullahi Abdurahman

Abstract :

A number of E. coli strains are recognized as important pathogens of colibacillosis in food animals (poultry and ruminants) and some of them can cause severe human diseases such as hemorrhagic colitis and hemolytic uremic syndrome. E. coli are Gram-negative, facultative anaerobic, rod-shaped and highly motile bacteria. A cross-sectional study was conducted from December 2020 to February 2021 to isolation and identification of E. coli as well as the antimicrobial susceptibility of E. coli isolated from raw milk. Biochemical tests methods were used to confirm E. coli and isolates were subjected to antimicrobial susceptibility test using the agar disc diffusion method. Out of 28 raw milk samples collected, 15 (53.6%) were found to be positive for E. coli. The presence of E. coli was higher in adults (77.8%) followed by (50%) in old cattle and least in young (28.6%) but it was furthermore the variation in the prevalence among age groups was not statistically Significant (P>0.05). Statistically significant difference was not observed (P>0.05) in the presence of E. coli among those animals with different body condition scores but the presence of E. coli was higher proportion in poor body condition cattle 6(60%), then in good body condition cattle 9(50%). The highest percentages of E. coli isolates were isolated from cows which have many parities 9(69.2%) and the lowest in cows with few parities 2(33.3%) and from cows with early lactation stage 7(58.3%). The antimicrobial susceptibility profile showed that the most frequently encountered form of resistance in all samples was resistance to Ampicillin (93.3%), followed by resistance to Amoxicillin (80%), resistance to Tetracycline (73.3%), resistance Chloramphenicol (33.3%) and resistance to kanamycin (20%). Milk samples were produced and handled under poor hygienic conditions, stored, and transported in inappropriate containers and under temperature abuse conditions leading to high health risk to the consumers. Relevant intervention program and awareness creation on best practice of milk handling should be conducted to minimize contamination of milk and milk products with pathogens of public health importance in Haramaya University dairy farm is necessary.

Keywords :

Antimicrobial   Susceptibility   E-coli   Raw  

Cite This Article :

ISOLATION, IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING OF ESCHERICHIA COLI ISOLETED FROM RAW COW MILKS OF BORENA BREED DAIRY FARMS IN HARAMAYA UNIVERSITY, EASTERN ETHIOPIA, Umer Seid, Chala Mohammed, Munera Ahmednur, Serkalem Haile, Abdullahi Abdurahman, INTERNATIONAL JOURNAL OF PURE MEDICAL RESEARCH : Volume-6 | Issue-3 | March-2021

Article :

View PDF Article No. : 1Number of Views : 791