Isolation of Lactic Acid Bacteria Producing Mannitol from Indonesian Cocoa Beans Fermentation
Anja Meryandini, Fariza Itsna Qurrota A Yun, Yopi
Mannitol is a polyol which is widely used in many industries. Lactic acid bacteria known as one of microorganism which produce mannitol in a certain condition. LABs are widely found in fermentation product. This research aims to isolate LAB producing mannitol from Indonesian cocoa beans fermentation as an alternative of comercial mannitol production. LAB isolated from 0 to 96 h of fermentation, screened by the clear zone formed in MRS medium contained CaCO3 1% and its catalase activity. Initial screening of 94 LAB isolates using TLC yield only ten LAB producing mannitol isolates. Further screening through HPLC analysis showed that LAB 4.32 produced the highest concentration of mannitol (1.873 g/L). Analysis of 16S rRNA genes revealed that LAB 4.32 was Lactobacillus plantarum. Cultivation of the LAB 4.32 in tofu whey with addition of (NH4)2SO4 1% (w/v) and fructose 5% (w/v) obtained the highest mannitol production up to 0.719 g/l.
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